Journal: The Journal of investigative dermatology

Article Title: Cellular, molecular and immunological characteristics of Langhans multinucleated giant cells programmed by IL-15

doi: 10.1016/j.jid.2020.01.026

Figure Lengend Snippet: Structure and formation of the LGCs. (a-h) The three-dimensional structure of MGCs from T-lep lesions. Series of cross-sectioned planes through an LGC from a T-lep lesions confirming that the LGC indeed is a three-dimensional sphere. (i) Each image represents one step of the 3D modeling of IL-15 derived LGCs. The first image shows the original confocal image, second to seventh images show different angles during the modeling and last image shows the 3-dimensional model of the nuclei ring. 3D modeling was performed using Imaris 8.0 software. (j) The formation of LGCs by IL-15 was measured by time lapse microscopy. Cell imaging capture was assessed each 10min in total of 3h by PerkinElmer Operetta (EPA, USA). Scale bars: a-h (100μm); i (10μm); j (50μm).

Article Snippet: Z slices images of all volume of the cell were scanned and a z stack total volume image of the cell were used to 3D modeling animation using Imaris 8.0 (Bitplane).

Techniques: Derivative Assay, Software, Time-lapse Microscopy, Imaging

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Buckling and twisting of advanced materials into morphable 3D mesostructures

doi: 10.1073/pnas.1901193116

Figure Lengend Snippet: Three-dimensional morphable mesostructures based on buckling and twisting with diverse geometries and materials. (A) Two-dimensional geometries, FEA predictions, and experimental images (optical) of a morphable 3D chiral, propeller structure. (B) Two-dimensional geometries, FEA predictions, and experimental images (optical) of a morphable 3D box structure. Shape I and shape II correspond to the 3D shapes after releasing of the stretching mode (prestrain from 100 to 40%) and releasing of the rotating mode (prestrain from 40 to 0%). (C) Two-dimensional geometries, FEA predictions (showing normalized displacements in z direction), and experimental images (optical) of an array of creased structures based on twisting-induced folding. (D) Two-level square substrate cut pattern, 2D geometries, FEA predictions, and experimental images (optical) of a structure with multiple creases. (E) FEA predictions and experimental images (optical) of 3 mesostructures constructed with diverse materials. Scale bars, 1 mm for structures in A–D; 2 mm for Cu structures (E, Top); and 500 μm for Si/SU8 and SMP structures (E, Middle and Bottom).

Article Snippet: The numerical simulation of 3D chiral microstructures used the commercial finite-element method package CST (Computer Simulation Technology GmbH).

Techniques: Construct